Analysis of fae and fhcD genes in Mono Lake, California

Appl Environ Microbiol. 2005 Dec;71(12):8949-53. doi: 10.1128/AEM.71.12.8949-8953.2005.

Abstract

Genes for two enzymes of the tetrahydromethanopterin-linked C(1) transfer pathway (fae and fhcD) were detected in hypersaline, hyperalkaline Mono Lake (California), via PCR amplification and analysis. Low diversity for fae and fhcD was noted, in contrast to the diversity previously detected in a freshwater lake, Lake Washington (Washington).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / genetics*
  • California
  • Carbon-Nitrogen Ligases / genetics*
  • Fresh Water / analysis
  • Fresh Water / microbiology*
  • Genetic Variation*
  • Gram-Negative Aerobic Rods and Cocci / classification
  • Gram-Negative Aerobic Rods and Cocci / genetics
  • Gram-Negative Aerobic Rods and Cocci / isolation & purification*
  • Methylomonas / classification
  • Methylomonas / genetics
  • Methylomonas / isolation & purification
  • Oxidation-Reduction
  • Oxygen / analysis
  • Phylogeny
  • Polymerase Chain Reaction / methods

Substances

  • Bacterial Proteins
  • Carbon-Nitrogen Ligases
  • Fae protein, Methylobacterium extorquens
  • Oxygen