Invasion of hematopoietic cells into the brain of amyloid precursor protein transgenic mice

J Neurosci. 2005 Nov 30;25(48):11125-32. doi: 10.1523/JNEUROSCI.2545-05.2005.

Abstract

The significance of the peripheral immune system in Alzheimer's disease pathogenesis remains controversial. To study the CNS invasion of hematopoietic cells in the course of cerebral amyloidosis, we used a green fluorescence protein (GFP)-bone marrow chimeric amyloid precursor protein transgenic mouse model (APP23 mice). No difference in the number of GFP-positive invading cells was observed between young APP23 mice and nontransgenic control mice. In contrast, in aged, amyloid-depositing APP23 mice, a significant increase in the number of invading ameboid-like GFP-positive cells was found compared with age-matched nontransgenic control mice. Interestingly, independent of the time after transplantation, only a subpopulation of amyloid deposits was surrounded by invading cells. This suggests that not all amyloid plaques are a target for invading cells or, alternatively, all amyloid plaques attract invading cells but only for a limited time, possibly at an early stage of plaque evolution. Immunological and ultrastructural phenotyping revealed that macrophages and T-cells accounted for a significant portion of these ameboid-like invading cells. Macrophages did not show evidence of amyloid phagocytosis at the electron microscopic level, and no obvious signs for T-cell-mediated inflammation or neurodegeneration were observed. The observation that hematopoietic cells invade the brain in response to cerebral amyloidosis may hold an unrecognized therapeutic potential.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid
  • Amyloid Neuropathies / metabolism
  • Amyloid Neuropathies / pathology
  • Amyloid Neuropathies / physiopathology
  • Amyloid beta-Protein Precursor / metabolism*
  • Animals
  • Bone Marrow Cells* / pathology
  • Bone Marrow Transplantation*
  • Brain / metabolism*
  • Brain Diseases / metabolism
  • Brain Diseases / pathology
  • Brain Diseases / physiopathology
  • Cell Movement*
  • Green Fluorescent Proteins
  • Hematopoiesis*
  • Macrophages
  • Male
  • Mice
  • Mice, Transgenic
  • Phagocytosis
  • T-Lymphocytes / pathology
  • Transplantation Chimera

Substances

  • Amyloid
  • Amyloid beta-Protein Precursor
  • Green Fluorescent Proteins