Abstract
In this study, we demonstrate the involvement of DNAM-1-triggering receptor and its ligands, poliovirus receptor (PVR) and Nectin-2, in natural killer (NK) cell-mediated lysis of dendritic cells (DCs). The surface expression of both ligands was up-regulated in DCs as compared to monocytes. It reached maximal densities after DC maturation induced by different stimuli including lipopolysaccharide (LPS), poly I:C, flagellin, and CD40L. Both immunohistochemical analysis and confocal microscopy revealed expression of DNAM-1 ligands by DCs in lymph nodes in which they were localized in the parafollicular T-cell region and surrounded the high endothelial venules. Remarkably, in cytolytic assays, DNAM-1 cooperated with NKp30 in the NK-mediated killing of both immature and mature DCs and the degree of contribution of DNAM-1 appeared to correlate with the surface densities of its specific ligands PVR and Nectin-2.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Differentiation, T-Lymphocyte / immunology*
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CD40 Ligand / pharmacology
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Cell Communication / drug effects
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Cell Communication / immunology*
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Cell Differentiation / drug effects
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Cell Differentiation / immunology
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Cells, Cultured
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Dendritic Cells / immunology*
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Humans
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Interleukin-2 Receptor beta Subunit
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Killer Cells, Natural / immunology*
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Ligands
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Lipopolysaccharides / pharmacology
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Membrane Glycoproteins / immunology
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Membrane Proteins / immunology*
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Natural Cytotoxicity Triggering Receptor 3
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Poly I-C / pharmacology
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Receptors, Immunologic / immunology
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Receptors, Interleukin-2 / immunology*
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Receptors, Virus / immunology*
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Up-Regulation / immunology
Substances
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Antigens, Differentiation, T-Lymphocyte
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CD226 antigen
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IL2RB protein, human
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Interleukin-2 Receptor beta Subunit
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Ligands
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Lipopolysaccharides
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Membrane Glycoproteins
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Membrane Proteins
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NCR3 protein, human
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Natural Cytotoxicity Triggering Receptor 3
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Receptors, Immunologic
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Receptors, Interleukin-2
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Receptors, Virus
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poliovirus receptor
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CD40 Ligand
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Poly I-C