Expression of the DNAM-1 ligands, Nectin-2 (CD112) and poliovirus receptor (CD155), on dendritic cells: relevance for natural killer-dendritic cell interaction

Blood. 2006 Mar 1;107(5):2030-6. doi: 10.1182/blood-2005-07-2696. Epub 2005 Nov 22.

Abstract

In this study, we demonstrate the involvement of DNAM-1-triggering receptor and its ligands, poliovirus receptor (PVR) and Nectin-2, in natural killer (NK) cell-mediated lysis of dendritic cells (DCs). The surface expression of both ligands was up-regulated in DCs as compared to monocytes. It reached maximal densities after DC maturation induced by different stimuli including lipopolysaccharide (LPS), poly I:C, flagellin, and CD40L. Both immunohistochemical analysis and confocal microscopy revealed expression of DNAM-1 ligands by DCs in lymph nodes in which they were localized in the parafollicular T-cell region and surrounded the high endothelial venules. Remarkably, in cytolytic assays, DNAM-1 cooperated with NKp30 in the NK-mediated killing of both immature and mature DCs and the degree of contribution of DNAM-1 appeared to correlate with the surface densities of its specific ligands PVR and Nectin-2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Differentiation, T-Lymphocyte / immunology*
  • CD40 Ligand / pharmacology
  • Cell Communication / drug effects
  • Cell Communication / immunology*
  • Cell Differentiation / drug effects
  • Cell Differentiation / immunology
  • Cells, Cultured
  • Dendritic Cells / immunology*
  • Humans
  • Interleukin-2 Receptor beta Subunit
  • Killer Cells, Natural / immunology*
  • Ligands
  • Lipopolysaccharides / pharmacology
  • Membrane Glycoproteins / immunology
  • Membrane Proteins / immunology*
  • Natural Cytotoxicity Triggering Receptor 3
  • Poly I-C / pharmacology
  • Receptors, Immunologic / immunology
  • Receptors, Interleukin-2 / immunology*
  • Receptors, Virus / immunology*
  • Up-Regulation / immunology

Substances

  • Antigens, Differentiation, T-Lymphocyte
  • CD226 antigen
  • IL2RB protein, human
  • Interleukin-2 Receptor beta Subunit
  • Ligands
  • Lipopolysaccharides
  • Membrane Glycoproteins
  • Membrane Proteins
  • NCR3 protein, human
  • Natural Cytotoxicity Triggering Receptor 3
  • Receptors, Immunologic
  • Receptors, Interleukin-2
  • Receptors, Virus
  • poliovirus receptor
  • CD40 Ligand
  • Poly I-C