Efficient enzymatic synthesis of 4-methylumbelliferyl N-acetyllactosaminide and 4-methylumbelliferyl sialyl N-acetyllactosaminides employing beta-D-galactosidase and sialyltransferases

Xiaoxiong Zeng; Yi Sun; Hirotaka Uzawa

doi:10.1007/s10529-005-1310-3

Efficient enzymatic synthesis of 4-methylumbelliferyl N-acetyllactosaminide and 4-methylumbelliferyl sialyl N-acetyllactosaminides employing beta-D-galactosidase and sialyltransferases

Biotechnol Lett. 2005 Oct;27(19):1461-5. doi: 10.1007/s10529-005-1310-3.

Authors

Xiaoxiong Zeng¹, Yi Sun, Hirotaka Uzawa

Affiliation

¹ Department of Biotechnology, College of Food Science and Technology, Nanjing Agricultural University, 210095, Weigang, Nanjing, Jiangsu, PR China. zengxx@njau.edu.cn

PMID: 16231217
DOI: 10.1007/s10529-005-1310-3

Abstract

4-Methylumbelliferyl N-acetyllactosaminide and 4-methylumbelliferyl sialyl N-acetyllactosaminides, which are used for the assay of sialytransferase, neuraminidase and fucosyltransferase, were synthesized, respectively, by the beta-D: -galactosidase from Bacillus circulans and by a recombinant rat alpha2,3-(N)-sialyltransferase or rat liver alpha2,6-(N)-sialyltransferase with CMP-N-acetylneuraminic acid as donor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylglucosamine / analogs & derivatives*
Acetylglucosamine / biosynthesis
Animals
Bacillus / enzymology
Hymecromone / analogs & derivatives*
Lactose / analogs & derivatives*
Lactose / biosynthesis
Liver / enzymology
Rats
Sialyltransferases / metabolism*
Umbelliferones / biosynthesis*
beta-Galactosidase / metabolism*

Substances

4-methylumbelliferyl sialyl N-acetyllactosaminide
Umbelliferones
4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside
Hymecromone
Sialyltransferases
beta-Galactosidase
Lactose
Acetylglucosamine