Objective: To detect the expression level of CpTI and HPT proteins in the seed of sck transgenic rice.
Methods: The seeds of T6 sck transgenic rice were detected by PCR. The CpTI and HPT proteins in the seeds, stems, roots and leaves of positive plants were detected by Western blot. The CpTI and HPT proteins in the seeds of positive plants and the control plants were detected by double sandwich ELISA.
Results: hpt and sck genes were found in T6 sck rice. The CpTI and HPT proteins were positive in the leaves, stems, and roots in Western blot analysis, and negative in the seeds. The content of CpTI protein is lower than LLD of double sandwich ELISA (< 14 ug/L), and HPT protein could not be detected.
Conclusion: The content of CpTI protein in the seeds of T6 sck rice is lower than LLD of double sandwich ELISA, and HPT protein could not be detected.