Development of cytochrome-c oxidase activity in rat heart: downregulation in newborn rats

Z Drahota; M Milerova; A Stieglerova; L Skarka; J Houstek; B Ostadal

doi:10.1385/CBB:43:1:087

Development of cytochrome-c oxidase activity in rat heart: downregulation in newborn rats

Cell Biochem Biophys. 2005;43(1):87-94. doi: 10.1385/CBB:43:1:087.

Authors

Z Drahota¹, M Milerova, A Stieglerova, L Skarka, J Houstek, B Ostadal

Affiliation

¹ Center for Integrated Genomics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic.

PMID: 16043886
DOI: 10.1385/CBB:43:1:087

Abstract

Cytochrome-c oxidase (COX) activity of the rat heart was two- to sevenfold activated when the membrane integrity was disrupted by hypotonic swelling, freezing-thawing, or a detergent, indicating that a large portion of COX capacity in intact mitochondria is not active. The effect of detergent was tested in heart mitochondria isolated from 1-, 5-, 15-, 29-, and 60-d-old rats; activation by detergent was up to 20-fold in 1-d-old animals, whereas in mitochondria from 60-d-old rats it was only 7-fold. Our data indicate that the rat heart exhibits significant developmental changes dependent on downregulation of COX activity in the neonatal period.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aging / drug effects
Aging / metabolism*
Animals
Animals, Newborn
Cells, Cultured
Detergents / administration & dosage
Down-Regulation / drug effects
Electron Transport Complex IV / metabolism*
Enzyme Activation / drug effects
Gene Expression Regulation, Developmental / drug effects
Gene Expression Regulation, Developmental / physiology*
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Enzymologic / physiology*
Heart / drug effects
Male
Mitochondria, Heart / drug effects
Mitochondria, Heart / enzymology*
Myocardium / enzymology*
Rats
Rats, Wistar

Substances

Detergents
Electron Transport Complex IV