Abstract
Purified QnrA blocked ciprofloxacin inhibition of topoisomerase IV, just as QnrA was previously found to prevent quinolone inhibition of DNA gyrase. With a gel displacement assay, tagged QnrA was shown to bind to topoisomerase IV and its subunits in a reaction that did not depend on the presence of DNA, quinolone, or ATP.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Anti-Infective Agents / pharmacology
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DNA Topoisomerase IV / metabolism*
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Drug Resistance, Bacterial / genetics
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Escherichia coli / drug effects
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Plasmids / genetics*
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Quinolones / pharmacology
Substances
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Anti-Infective Agents
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Escherichia coli Proteins
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Qnr protein, E coli
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Quinolones
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DNA Topoisomerase IV