Chiral separation of anticholinergic drug enantiomers in nonaqueous capillary electrophoresis

Guohua Du; Shuzhen Zhang; Jianwei Xie; Bohua Zhong; Keliang Liu

doi:10.1016/j.chroma.2005.03.009

Chiral separation of anticholinergic drug enantiomers in nonaqueous capillary electrophoresis

J Chromatogr A. 2005 May 13;1074(1-2):195-200. doi: 10.1016/j.chroma.2005.03.009.

Authors

Guohua Du¹, Shuzhen Zhang, Jianwei Xie, Bohua Zhong, Keliang Liu

Affiliation

¹ Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.

PMID: 15941055
DOI: 10.1016/j.chroma.2005.03.009

Abstract

Nonaqueous capillary electrophoretic (NACE) method for the separation of nine structurally similar chiral anticholinergic drugs was developed. The eight drug enantiomers were separated on baseline within 18 min using 20mM phosphoric acid and 10 mM NaOH, containing 10 mM heptakis(2,3-dimethyl-6-sulfato)-4beta-cyclodextrin (HDMS-beta-CD) in methanol. The results were compared with those obtained in the high performance liquid chromatography system.

Publication types

Comparative Study

MeSH terms

Cholinergic Antagonists / isolation & purification*
Chromatography, High Pressure Liquid
Electrophoresis, Capillary / methods*
Hydrogen-Ion Concentration
Stereoisomerism
beta-Cyclodextrins

Substances

Cholinergic Antagonists
beta-Cyclodextrins
heptakis(2,3-dimethyl-6-sulfato)cyclodextrin