Identification of a progenitor of the CTX-M-9 group of extended-spectrum beta-lactamases from Kluyvera georgiana isolated in Guyana

Antimicrob Agents Chemother. 2005 May;49(5):2112-5. doi: 10.1128/AAC.49.5.2112-2115.2005.

Abstract

Chromosomal beta-lactamase genes (bla(KLUY)) from six Kluyvera georgiana strains isolated in Guyana were cloned and expressed in Escherichia coli. KLUY-1 exhibited 100% amino acid identity with the extended-spectrum beta-lactamase CTX-M-14. We also show that a 2.7-kb Kluyvera chromosomal region exhibits 99% nucleotide identity to a portion of In60 that includes bla(CTX-M-9).

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents / pharmacology
  • Chromosomes, Bacterial / genetics
  • Cloning, Molecular
  • Deoxyribonuclease EcoRI / chemistry
  • Escherichia coli / genetics
  • Escherichia coli Proteins / genetics*
  • Guyana
  • Humans
  • Kluyvera / drug effects
  • Kluyvera / enzymology*
  • Kluyvera / genetics*
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • beta-Lactamases / genetics*

Substances

  • Anti-Bacterial Agents
  • Escherichia coli Proteins
  • Deoxyribonuclease EcoRI
  • CTX-M-9 protein, E coli
  • beta-Lactamases

Associated data

  • GENBANK/AY623932
  • GENBANK/AY623933
  • GENBANK/AY623934
  • GENBANK/AY623935
  • GENBANK/AY824948