A method for determining intracellular concentrations of enzyme substrates from a combination of competitive inhibition and mutagenesis studies

Anal Biochem. 2005 Feb 15;337(2):351-3. doi: 10.1016/j.ab.2004.10.010.

Authors

Robert A Copeland¹, Lusong Luo, Kurt R Auger, Pearl S Huang

Affiliation

¹ Department of Enzymology and Mechanistic Pharmacology and Department of Oncology Biology, GlaxoSmithKline, 1250 S. Collegeville Road, Collegeville, PA 19426, USA. robert.a.copeland@gsk.com

PMID: 15691518
DOI: 10.1016/j.ab.2004.10.010

No abstract available

MeSH terms

Binding, Competitive
Enzyme Inhibitors / metabolism
Enzyme Inhibitors / pharmacology
Enzymes / genetics
Enzymes / metabolism*
Intracellular Space / enzymology
Intracellular Space / metabolism*
Kinetics
Ligands
Mutagenesis*

Substances

Enzyme Inhibitors
Enzymes
Ligands