Immunotherapy with fragmented Mycobacterium tuberculosis cells increases the effectiveness of chemotherapy against a chronical infection in a murine model of tuberculosis

Vaccine. 2005 Feb 3;23(11):1393-8. doi: 10.1016/j.vaccine.2004.09.008.

Abstract

Reduction of colony forming units by rifampicin-isoniazid therapy given 9-17 weeks post-infection was made more pronounced by immunotherapy with a vaccine made of fragmented Mycobacterium tuberculosis cells detoxified and liposomed (RUTI), given on weeks 17, 19 and 21 post-infection, in the murine model of tuberculosis in C57BL/6 and DBA/2 inbred strains. RUTI triggered a Th1/Th2 response, as demonstrated by the production of IgG1, IgG2a and IgG3 antibodies against a wide range of peptides. The histological analysis did not show neither eosinophilia nor necrosis, and granulomatous infiltration was only slightly increased in C57BL/6 mice when RUTI was administered intranasally.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Bacterial / blood
  • Antitubercular Agents / therapeutic use*
  • Colony Count, Microbial
  • Combined Modality Therapy
  • Disease Models, Animal
  • Female
  • Immunoglobulin G / blood
  • Immunotherapy
  • Interferon-gamma / analysis
  • Isoniazid / therapeutic use*
  • Lung / microbiology
  • Lung / pathology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Mycobacterium tuberculosis / immunology*
  • Rifampin / therapeutic use*
  • Spleen / microbiology
  • Tuberculosis / drug therapy
  • Tuberculosis / therapy*
  • Tuberculosis Vaccines / therapeutic use*
  • Tumor Necrosis Factor-alpha / analysis

Substances

  • Antibodies, Bacterial
  • Antitubercular Agents
  • Immunoglobulin G
  • Tuberculosis Vaccines
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma
  • Isoniazid
  • Rifampin