Abstract
Gas chromatographic/mass spectroscopic and chiral analysis showed the presence of enzymatically derived 8,9-, 11,12- and 14,15-EET in rat plasma (2.8:1:3.4 molar ratio, respectively; 10.2 +/- 0.4 ng total EET/ml plasma). Greater than 90% of the plasma EETs was esterified to the phospholipids of circulating lipoproteins. The lipoprotein fraction with the highest EET concentration was LDL (8.1 +/- 0.9 ng/mg of protein) followed by HDL and VLDL (3.5 +/- 0.1 and 1.9 +/- 0.3 ng/mg of protein, respectively). In light of the biological activities of the EETs, these results suggest a potential systemic function for the cytochrome P-450 epoxygenase.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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8,11,14-Eicosatrienoic Acid / analogs & derivatives*
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8,11,14-Eicosatrienoic Acid / blood
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Animals
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Cytochrome P-450 CYP2J2
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Cytochrome P-450 Enzyme System / metabolism*
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Gas Chromatography-Mass Spectrometry
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Isomerism
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Lipoproteins / blood*
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Lipoproteins, HDL / blood
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Lipoproteins, LDL / blood
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Lipoproteins, VLDL / blood
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Male
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Oxygenases / metabolism*
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Rats
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Rats, Inbred Strains
Substances
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Lipoproteins
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Lipoproteins, HDL
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Lipoproteins, LDL
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Lipoproteins, VLDL
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11,12-epoxy-5,8,14-eicosatrienoic acid
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8,9-epoxyeicosatrienoic acid
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14,15-epoxy-5,8,11-eicosatrienoic acid
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Cytochrome P-450 Enzyme System
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Oxygenases
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Cytochrome P-450 CYP2J2
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8,11,14-Eicosatrienoic Acid