Optimizing RNA extraction yield from whole blood for microarray gene expression analysis

Jian Wang; John F Robinson; Hafiz M R Khan; David E Carter; James McKinney; Brooke A Miskie; Robert A Hegele

doi:10.1016/j.clinbiochem.2004.03.013

Optimizing RNA extraction yield from whole blood for microarray gene expression analysis

Clin Biochem. 2004 Sep;37(9):741-4. doi: 10.1016/j.clinbiochem.2004.03.013.

Authors

Jian Wang¹, John F Robinson, Hafiz M R Khan, David E Carter, James McKinney, Brooke A Miskie, Robert A Hegele

Affiliation

¹ Vascular Biology Research Group and London Regional Genomics Centre, Robarts Research Institute and University of Western Ontario, London, ON, N6A 5K8, Canada.

PMID: 15329310
DOI: 10.1016/j.clinbiochem.2004.03.013

Abstract

Objectives: Microarray analysis of gene expression profiles of blood leukocytes has many potential clinical and research applications.

Design and methods: We used the PAXgene Blood RNA System to prepare RNA from the whole blood of normal volunteers using two incubation times followed by gene expression profiling using the Affymetrix HU133A GeneChip.

Conclusions: Longer incubation gave a significantly higher RNA yield and samples that were satisfactory for microarray analysis, with excellent pairwise correlations between replicates.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Profiling / methods*
Gene Expression Profiling / standards
Humans
Oligonucleotide Array Sequence Analysis / methods*
Oligonucleotide Array Sequence Analysis / standards
RNA / blood*
RNA / genetics*

Substances

RNA