In this paper, the accumulation and translocation of lead in soil-crop ecosystems were studied through fertilization with chemical fertilizer, manure and bio-fertilizer. The results showed that the soil total Pb content was decreased with soil depth. It was 11.14 and 9.37 mg x kg(-1) in 0-15 and 15-30 cm after harvest, respectively. Rice root absorbed lead from soil, and transferred it to stem-leaf and grain. The Pb content in rice plant was root>stem and leaf>grain, which was increased with rice growth. The grains of 606-14 and Liao 294 contained 0. 008 and 0.007 mg x kg(-1) of Pb, respectively, which is under the standard of green food. Variance analysis indicated that the grain yield of different varieties had a significant variance, but no significant variance was found among different treatments. It could be summarized that manure or bio-fertilizer could be the substitute of chemical fertilizer in producing rice.