Crystallization of a calcium-binding EGF-like domain

Z Rao; P A Handford; V Knott; M Mayhew; G G Brownlee; D Stuart

doi:10.1107/S0907444994009881

Crystallization of a calcium-binding EGF-like domain

Acta Crystallogr D Biol Crystallogr. 1995 May 1;51(Pt 3):402-3. doi: 10.1107/S0907444994009881.

Authors

Z Rao¹, P A Handford, V Knott, M Mayhew, G G Brownlee, D Stuart

Affiliation

¹ Laboratory of Molecular Biophysics and Oxford Centre for Molecular Sciences, England.

PMID: 15299311
DOI: 10.1107/S0907444994009881

Abstract

Crystals of a calcium-binding epidermal growth factor (EGF)-like domain of human clotting factor IX suitable for X-ray diffraction analysis have been obtained by vapour diffusion (sitting drop) against 48% PEG 400. The crystals belong to the tetragonal space group P4(3)2(1)2, with unit-cell dimensions a = b = 40.3, c = 98.2 A. The crystals diffract beyond 1.5 A resolution and are relatively stable in the X-ray beam. This is the first reported crystallization of a calcium-binding EGF-like domain.