Multiplex PCR assay for rapid detection and genotyping of Helicobacter pylori directly from biopsy specimens

J Clin Microbiol. 2004 Jun;42(6):2821-4. doi: 10.1128/JCM.42.6.2821-2824.2004.

Abstract

We developed and evaluated a simple, novel multiplex PCR assay for rapid detection of Helicobacter pylori infection and for the determination of vacA and cagA genotypes directly from gastric biopsy specimens. This assay did not require culturing of strains or extraction of DNA from biopsy samples. This multiplex PCR assay would be of particularly great value for laboratories in developing countries.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Bacterial / genetics
  • Bacterial Proteins / genetics
  • Biopsy
  • Genotype
  • Helicobacter Infections / diagnosis*
  • Helicobacter pylori / classification
  • Helicobacter pylori / genetics
  • Helicobacter pylori / isolation & purification*
  • Humans
  • Polymerase Chain Reaction / methods*

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • VacA protein, Helicobacter pylori
  • cagA protein, Helicobacter pylori