Characterisation of a tetrasaccharide released on mild acid hydrolysis of LPS from two rough strains of Shewanella species representing different DNA homology groups

Anne L Moule; Lesley Galbraith; Andrew D Cox; Stephen G Wilkinson

doi:10.1016/j.carres.2004.01.017

Characterisation of a tetrasaccharide released on mild acid hydrolysis of LPS from two rough strains of Shewanella species representing different DNA homology groups

Carbohydr Res. 2004 Apr 28;339(6):1185-8. doi: 10.1016/j.carres.2004.01.017.

Authors

Anne L Moule¹, Lesley Galbraith, Andrew D Cox, Stephen G Wilkinson

Affiliation

¹ Department of Chemistry, University of Hull, Hull HU6 7RX, UK.

PMID: 15063209
DOI: 10.1016/j.carres.2004.01.017

Abstract

A reducing tetrasaccharide of the following structure was released by mild acid hydrolysis of R-type LPS from Shewanella putrefaciens strains NCIMB 10472 and 10473. The same tetrasaccharide containing acetal-linked open-chain GalNAc is present in the core region of LPS from S. oneidensis strain MR-1 and may be characteristic of genomic groups II and III of S. putrefaciens and related strains. (1S)-d-GalaNAc-(1-->4,6)-alpha-d-Galp-(1-->6)-alpha-d-Galp-(1-->3)-d-Gal.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylgalactosamine / chemistry
Carbohydrate Conformation
Carbohydrate Sequence
DNA / chemistry*
Hydrolysis
Lipopolysaccharides / chemistry*
Magnetic Resonance Spectroscopy
Mass Spectrometry
Models, Chemical
Molecular Sequence Data
Oligosaccharides / chemistry
Polysaccharides / chemistry
Shewanella
Species Specificity

Substances

Lipopolysaccharides
Oligosaccharides
Polysaccharides
DNA
Acetylgalactosamine