ESF1 is required for 18S rRNA synthesis in Saccharomyces cerevisiae

Wen-Tao Peng; Nevan J Krogan; Dawn P Richards; Jack F Greenblatt; Timothy R Hughes

doi:10.1093/nar/gkh518

ESF1 is required for 18S rRNA synthesis in Saccharomyces cerevisiae

Nucleic Acids Res. 2004 Mar 31;32(6):1993-9. doi: 10.1093/nar/gkh518. Print 2004.

Authors

Wen-Tao Peng¹, Nevan J Krogan, Dawn P Richards, Jack F Greenblatt, Timothy R Hughes

Affiliation

¹ Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario M5G 1L6, Canada.

Abstract

We report that Esf1p (Ydr365cp), an essential, evolutionarily conserved nucleolar protein, is required for the biogenesis of 18S rRNA in Saccharomyces cerevisiae. Depletion of Esf1p resulted in delayed processing of 35S precursor and a striking loss of 18S rRNA. Esf1p physically associated with ribosomal proteins and proteins involved in 18S rRNA biogenesis. Consistent with its role in 18S rRNA biogenesis, Esf1p also physically associated with U3 and U14 snoRNAs, but did not appear to be a core component of the SSU processome. These data indicate that Esf1p plays a direct role in early pre-rRNA processing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Conserved Sequence
Molecular Sequence Data
Nuclear Proteins / genetics
Nuclear Proteins / physiology*
RNA Precursors / chemistry
RNA Precursors / metabolism
RNA, Ribosomal, 18S / biosynthesis*
RNA, Ribosomal, 18S / chemistry
RNA, Small Nucleolar / metabolism
Ribosomal Proteins / metabolism
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / physiology*
Sequence Alignment

Substances

ESF1 protein, S cerevisiae
Nuclear Proteins
RNA Precursors
RNA, Ribosomal, 18S
RNA, Small Nucleolar
Ribosomal Proteins
Saccharomyces cerevisiae Proteins