Disruption of basement membrane, extracellular matrix metalloproteinases and E-cadherin in renal-cell carcinoma

Anticancer Res. 2003 Nov-Dec;23(6D):5005-10.

Abstract

A retrospective study was performed to determine the prognostic value of Basement Membrane (BM) integrity, Matrix Metalloproteinases (MMPs) and E-Cadherin expression in renal cell carcinoma (RCC).

Materials and methods: An immunohistochemical study on laminin and collagen IV, MMPs 1 and 2, and E-Cadherin was carried out on 71 RCCs. BM fragmentation was considered taking 75% as a cut-off. MMP 1 and MMP2 immunostaining, as well as E-Cadherin was considered taking 25% as a cut-off.

Results: An inverse relationship was seen between E-Cadherin with laminin, collagen IV and MMPs. More than 75% loss of laminin, collagen IV and E-Cadherin, as well as higher expression of MMPs, were associated with symptoms, tumoral size and worse grade. Loss of collagen IV and E-Cadherin were of prognostic value.

Conclusion: Both BM and E-Cadherin are good prognostic markers. MMPs patterns show a relationship between BM proteins and E-Cadherin, but evaluation is more time-consuming and provide no better prognostication; consequently they are not useful in routine clinical applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basement Membrane / enzymology
  • Basement Membrane / metabolism
  • Basement Membrane / pathology
  • Biomarkers, Tumor / biosynthesis*
  • Cadherins / biosynthesis
  • Carcinoma, Renal Cell / enzymology
  • Carcinoma, Renal Cell / metabolism*
  • Carcinoma, Renal Cell / pathology
  • Collagen Type IV / biosynthesis
  • Female
  • Humans
  • Immunohistochemistry
  • Kidney Neoplasms / enzymology
  • Kidney Neoplasms / metabolism*
  • Kidney Neoplasms / pathology
  • Laminin / biosynthesis
  • Male
  • Matrix Metalloproteinase 1 / biosynthesis
  • Matrix Metalloproteinase 2 / biosynthesis
  • Membrane Proteins / biosynthesis*
  • Middle Aged
  • Neoplasm Proteins / biosynthesis*
  • Neoplasm Staging
  • Prognosis

Substances

  • Biomarkers, Tumor
  • Cadherins
  • Collagen Type IV
  • Laminin
  • Membrane Proteins
  • Neoplasm Proteins
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 1