Synthesis of 7'-[123I]iodo-D-luciferin for in vivo studies of firefly luciferase gene expression

Bioorg Med Chem Lett. 2004 Mar 8;14(5):1161-3. doi: 10.1016/j.bmcl.2003.12.066.

Abstract

D-(-)-2-(6'-hydroxy-7'-[(123)I]iodobenzothiazolyl)-delta(2)-thiazoline-4-caroxylic acid (7'-[(123)I]iodo-D-luciferin) was synthesized as a novel reporter probe for in vivo studies of firefly luciferase gene expression. 7'-Iodo-D-luciferin, a nonradioactive standard, was synthesized and showed the binding property (K(M)=4.28 microM) similar to that of D-luciferin (2.53 microM) for firefly luciferase in luminescence assay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Coleoptera
  • Firefly Luciferin / chemical synthesis*
  • Firefly Luciferin / genetics
  • Gene Expression Regulation, Enzymologic / physiology*
  • Iodine Radioisotopes / chemistry*
  • Luciferases / biosynthesis*
  • Luciferases / genetics

Substances

  • Iodine Radioisotopes
  • Firefly Luciferin
  • Luciferases