The use of a spectrophotometric assay to study the interaction of S-adenosylmethionine synthetase with methionine analogues

Anal Biochem. 1992 Nov 15;207(1):68-72. doi: 10.1016/0003-2697(92)90501-w.

Abstract

We have developed a continuous spectrophotometric assay for S-adenosylmethionine synthetase and, using this assay, have examined the interaction of five potential inhibitors with the E. coli enzyme. S-Vinylhomocysteine and S-allylhomocysteine were found to be substrates, while S-(methanethio)cysteine and S-(methanethio)homocysteine were found to be competitive inhibitors. S-Cyanohomocysteine is neither a substrate nor an inhibitor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / analysis
  • Bacterial Proteins / antagonists & inhibitors*
  • Binding, Competitive
  • Drug Stability
  • Escherichia coli / enzymology
  • Ethionine / analogs & derivatives
  • Ethionine / metabolism
  • Ethionine / pharmacology
  • Kinetics
  • Methionine / analogs & derivatives*
  • Methionine / metabolism
  • Methionine / pharmacology
  • Methionine Adenosyltransferase / analysis
  • Methionine Adenosyltransferase / antagonists & inhibitors*
  • Spectrophotometry
  • Sulfonium Compounds / metabolism

Substances

  • Bacterial Proteins
  • Sulfonium Compounds
  • vinthionine
  • Methionine
  • Methionine Adenosyltransferase
  • Ethionine