Abstract
Structure-activity studies around the urea linkage in BMS-193885 (4a) identified the cyanoguanidine moiety as an effective urea replacement in a series of dihydropyridine NPY Y(1) receptor antagonists. In comparison to urea 4a (K(i)=3.3 nM), cyanoguanidine 20 (BMS-205749) displayed similar binding potency at the Y(1) receptor (K(i)=5.1 nM) and full functional antagonism (K(b)=2.6 nM) in SK-N-MC cells. Cyanoguanidine 20 also demonstrated improved permeability properties in Caco-2 cells in comparison to urea 4a (43 vs 19 nm/s).
MeSH terms
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Caco-2 Cells
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Cell Membrane Permeability / drug effects
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Cells, Cultured
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Dihydropyridines / chemistry
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Dihydropyridines / pharmacology
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Drug Evaluation, Preclinical / methods
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Guanidines / chemistry
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Humans
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Phenylurea Compounds / chemistry
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Phenylurea Compounds / pharmacology
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Receptors, Neuropeptide Y / antagonists & inhibitors*
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Receptors, Neuropeptide Y / metabolism
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Stereoisomerism
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Structure-Activity Relationship
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Urea / chemistry*
Substances
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BMS 193885
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Dihydropyridines
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Guanidines
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Phenylurea Compounds
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Receptors, Neuropeptide Y
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neuropeptide Y-Y1 receptor
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1,4-dihydropyridine
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Urea
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dicyandiamido