PCR-based genotyping of Mycobacterium tuberculosis with new GC-rich repeated sequences and IS6110 inverted repeats used as primers

Roman Kotłowski; Isdore Chola Shamputa; Nabil Abdullah El Aila; Anna Sajduda; Leen Rigouts; Armand van Deun; Françoise Portaels

doi:10.1128/JCM.42.1.372-377.2004

PCR-based genotyping of Mycobacterium tuberculosis with new GC-rich repeated sequences and IS6110 inverted repeats used as primers

J Clin Microbiol. 2004 Jan;42(1):372-7. doi: 10.1128/JCM.42.1.372-377.2004.

Authors

Roman Kotłowski¹, Isdore Chola Shamputa, Nabil Abdullah El Aila, Anna Sajduda, Leen Rigouts, Armand van Deun, Françoise Portaels

Affiliation

¹ Mycobacteriology Unit, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium.

Abstract

In the present study we attempted to develop a PCR-based epidemiological tool for the differentiation of Mycobacterium tuberculosis isolates. Use of the designed primers Mtb1 (5'-CCG-GCG-GGG-CCG-GCG-G) and Mtb2 (5'-CGG-CGG-CAA-CGG-CGG-C) targeting frequently repeated 16-bp sequences in combination with primers sited at the inverted repeats flanking IS6110 allowed differentiation of M. tuberculosis isolates.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Fingerprinting
DNA Primers
DNA Transposable Elements*
Genotype
Mycobacterium tuberculosis / classification
Mycobacterium tuberculosis / genetics*
Polymerase Chain Reaction / methods*
Repetitive Sequences, Nucleic Acid*

Substances

DNA Primers
DNA Transposable Elements