A fluorescent coagulation assay for thrombin using a fibre optic evanescent wave sensor

Steven R Garden; George J Doellgast; Kenneth S Killham; Norval J C Strachan

doi:10.1016/j.bios.2003.08.002

A fluorescent coagulation assay for thrombin using a fibre optic evanescent wave sensor

Biosens Bioelectron. 2004 Feb 15;19(7):737-40. doi: 10.1016/j.bios.2003.08.002.

Authors

Steven R Garden¹, George J Doellgast, Kenneth S Killham, Norval J C Strachan

Affiliation

¹ Department of Plant, Soil Science, School of Biological Sciences, Cruickshank Building, University of Aberdeen, Aberdeen AB24 3UU, UK.

PMID: 14709392
DOI: 10.1016/j.bios.2003.08.002

Abstract

A fibre optic evanescent wave sensor is used for the rapid detection of thrombin. Coagulation of solution phase fluorescently labelled fibrinogen to unlabelled fibrinogen bound to the surface of the fibre optic is observed in real time by the evanescent wave sensor. Thrombin concentrations down to 0.01 NIHml(-1) are detectable within 5 min. The potential application of this technique for rapid amplified immunosensing is discussed.

Publication types

Evaluation Study

MeSH terms

Biosensing Techniques / instrumentation*
Biosensing Techniques / methods
Blood Coagulation Tests / instrumentation*
Blood Coagulation Tests / methods
Equipment Design
Equipment Failure Analysis
Fiber Optic Technology / instrumentation*
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Fluorescence / instrumentation*
Spectrometry, Fluorescence / methods
Thrombin / analysis*

Substances

Thrombin