Impact of c-Met expression on angiogenesis in soft tissue sarcomas: correlation to microvessel-density

J Cancer Res Clin Oncol. 2003 Jul;129(7):415-22. doi: 10.1007/s00432-003-0456-4. Epub 2003 Jul 9.

Abstract

Purpose and methods: The c-Met protein is significant for oncogenesis and angiogenesis within the c-Met/HGF/SF-mediator system. The aim of this study was the analysis of c-Met immunoexpression/-synthesis and microvessel density as parameters for angiogenesis and prognosis in 115 soft tissue sarcomas.

Results: C-Met could be detected by immunohistochemistry in 87% of sarcomas. In all, 60.9% of cases exhibited absent or faint expression of c-Met protein, and 39.1% high expression of c-Met protein with a correlation between tumor grading and c-Met immunoexpression. Using in situ hybridization with detection of c-met-mRNA-transcripts, c-Met protein synthesis within tumor cells could be demonstrated. A statistically significant correlation between c-Met immunoexpression and tumor microvessel density was found. No prognostic value of c-Met expression and microvessel density could be detected in 56 patients with clinical follow-up ( P=0.8506 and P=0.9329 for disease-free survival).

Conclusions: The results underline a role of c-Met as an oncoprotein in soft tissue sarcomas with correlation between immunoexpression and grading. The statistically significant correlation between c-Met expression and microvessel density (as a parameter of tumor angiogenesis) suggests an angiogenic function of the c-Met/HGF/SF mediator system in malignant mesenchymal tumors.

MeSH terms

  • Adult
  • Aged
  • Female
  • Humans
  • Male
  • Middle Aged
  • Neovascularization, Pathologic*
  • Prognosis
  • Proto-Oncogene Proteins c-met / metabolism*
  • Sarcoma / blood supply*
  • Sarcoma / metabolism*
  • Sarcoma / pathology

Substances

  • Proto-Oncogene Proteins c-met