Novel photocleavable universal support for oligonucleotide synthesis

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1403-6. doi: 10.1081/NCN-120022996.

Abstract

A novel photocleavable universal support for the automated solid phase synthesis of oligonucleotides is described. The linker between the growing oligonucleotide chain and CPG support contains a nucleophilic amine protected with a photocleavable group. On exposure to UV light, this group is detached and the free amine affords cleavage of the oligonucleotide from the support. The use of long wavelength UV light avoids damage to the DNA.

MeSH terms

  • Base Sequence
  • DNA Damage
  • Indicators and Reagents
  • Molecular Structure
  • Oligodeoxyribonucleotides / chemical synthesis*
  • Oligodeoxyribonucleotides / radiation effects*
  • Ultraviolet Rays*

Substances

  • Indicators and Reagents
  • Oligodeoxyribonucleotides