Subcellular organization of Streptomyces aureofaciens and overproduction of chlortetracycline

Z Hostálek; J Novotná; V Starý; L Kalachová; J Vorísek

doi:10.1016/0734-9750(90)90009-z

Subcellular organization of Streptomyces aureofaciens and overproduction of chlortetracycline

Biotechnol Adv. 1990;8(1):131-9. doi: 10.1016/0734-9750(90)90009-z.

Authors

Z Hostálek¹, J Novotná, V Starý, L Kalachová, J Vorísek

Affiliation

¹ Institute of Microbiology, Czechoslovak Academy of Sciences, Vídenská 1083, 142 20 4, Prague, Czechoslovakia

PMID: 14545907
DOI: 10.1016/0734-9750(90)90009-z

Abstract

Biosynthesis of chlortetracycline is localized differently under low- and high-production conditions (standard low-production strain and its high-production variant). The experimental evidence was based on the assay of anhydrotetracycline oxygenase in subcellular fractions, ultracytochemical localization and electron-probe X-ray microanalysis of the product in the mycelium. Overproduction of chlortetracycline is closely associated with compartmentation of biosynthetic enzymes and with an efficient export of the antibiotic out of the cell.