Roles of FcgammaRIIB in nasal eosinophilia and IgE production in murine allergic rhinitis

Am J Respir Crit Care Med. 2004 Jan 1;169(1):105-12. doi: 10.1164/rccm.200302-239OC. Epub 2003 Oct 2.

Abstract

The low-affinity IgG Fc receptor, FcgammaRIIB, displays inhibitory potential in experimental models such as autoimmune diseases. However, whether this receptor is involved in the onset of allergic diseases remains unknown. This study examines the role of FcgammaRIIB in the initiation of allergic rhinitis in mice. Repeated intranasal sensitization with Schistosoma mansoni egg antigen (SEA) induced SEA-specific IgE and marked nasal eosinophilia in high-responder BALB/c mice. FcgammaRIIB gene-deficient (-/-) BALB/c mice displayed severe eosinophilia compared with that of wild-type counterparts. However, FcgammaRIIB -/- mice conversely produced less SEA-specific IgE. The production of interleukin (IL)-4 but not of IL-5 or IFN-gamma by nasal mononuclear cells was also decreased in FcgammaRIIB -/- mice, suggesting that the exacerbation of nasal eosinophila in FcgammaRIIB -/- mice is independent of the local IL-5 levels. The findings in low responder C57BL/6 mice were similar. In addition, nasal eosinophilia in FcgammaRIIB -/- mice passively sensitized with SEA was exacerbated, and conversely, specific IgE production was inhibited after a nasal challenge. These results suggest that FcgammaRIIB plays a regulatory role in the initiation of allergic rhinitis that is independent of either mouse strain or type of sensitization.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biopsy, Needle
  • Disease Models, Animal
  • Eosinophilia / immunology*
  • Eosinophilia / physiopathology
  • Female
  • Immunization
  • Immunoglobulin E / analysis
  • Immunoglobulin E / biosynthesis*
  • Immunohistochemistry
  • Interferon-gamma / immunology
  • Interleukin-4 / immunology
  • Interleukin-5 / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Nasal Mucosa / immunology
  • Nasal Mucosa / pathology*
  • Receptors, IgE / immunology
  • Receptors, IgG / immunology*
  • Rhinitis, Allergic, Perennial / immunology*
  • Rhinitis, Allergic, Perennial / physiopathology
  • Sensitivity and Specificity

Substances

  • Interleukin-5
  • Receptors, IgE
  • Receptors, IgG
  • Interleukin-4
  • Immunoglobulin E
  • Interferon-gamma