Bone-targeted Src kinase inhibitors: novel pyrrolo- and pyrazolopyrimidine analogues

Bioorg Med Chem Lett. 2003 Sep 15;13(18):3063-6. doi: 10.1016/s0960-894x(03)00647-4.

Abstract

Src tyrosine kinase is a therapeutic target for bone diseases that has been validated by gene knockout studies. Furthermore, in vitro cellular studies implicate that Src has a positive regulatory role in osteoclasts and a negative regulatory role in osteoblasts. The potential use of Src inhibitors for osteoporosis therapy has been previously shown by novel bone-targeted ligands of the Src SH2 (e.g., AP22408) and non-bone-targeted, ATP-based inhibitors of Src kinase. Significant to this study, compounds 2-12 exemplify novel analogues of known pyrrolopyrimidine and pyrazolopyrimidine template-based Src kinase inhibitors that incorporate bone-targeting group modifications designed to provide tissue (bone) selectivity and diminished side effects. Accordingly, we report here the structure-based design, synthetic chemistry and biological testing of these compounds and proof-of-concept studies thereof.

MeSH terms

  • Adenosine Triphosphate / analogs & derivatives*
  • Animals
  • Bone Diseases / drug therapy
  • Drug Design*
  • Enzyme Inhibitors / chemical synthesis
  • Enzyme Inhibitors / pharmacology
  • Humans
  • Inhibitory Concentration 50
  • Models, Molecular
  • Osteoporosis / drug therapy*
  • Purines / chemical synthesis
  • Purines / pharmacology
  • Pyrimidines / chemical synthesis*
  • Pyrimidines / pharmacology
  • Structure-Activity Relationship
  • src-Family Kinases / antagonists & inhibitors*

Substances

  • Enzyme Inhibitors
  • Purines
  • Pyrimidines
  • Adenosine Triphosphate
  • src-Family Kinases