Molecular characterization of isolates of waterborne Cryptosporidium spp. collected during an outbreak of gastroenteritis in South Burgundy, France

J Clin Microbiol. 2003 Jun;41(6):2690-3. doi: 10.1128/JCM.41.6.2690-2693.2003.

Abstract

In September 2001, a waterborne outbreak of gastroenteritis occurred in eastern France. Of 31 fecal samples from symptomatic individuals, 19 tested positive for Cryptosporidium with two PCRs targeting the Hsp70 and the 18S rRNA genes of CRYPTOSPORIDIUM: Sequencing of the PCR fragments produced sequences identical to that of Cryptosporidium parvum genotype 1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cryptosporidiosis / epidemiology
  • Cryptosporidiosis / parasitology
  • Cryptosporidium parvum / classification*
  • Cryptosporidium parvum / genetics*
  • Cryptosporidium parvum / isolation & purification
  • DNA, Ribosomal / analysis
  • Disease Outbreaks*
  • Feces / parasitology
  • France
  • Gastroenteritis / epidemiology*
  • Gastroenteritis / parasitology*
  • HSP70 Heat-Shock Proteins / genetics
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 18S / genetics
  • Sequence Analysis, DNA
  • Water / parasitology*

Substances

  • DNA, Ribosomal
  • HSP70 Heat-Shock Proteins
  • RNA, Ribosomal, 18S
  • Water

Associated data

  • GENBANK/AY177391