Prenatal diagnosis of congenital human cytomegalovirus infection in amniotic fluid by nucleic acid sequence-based amplification assay

M Grazia Revello; Daniele Lilleri; Maurizio Zavattoni; Milena Furione; Jaap Middeldorp; Giuseppe Gerna

doi:10.1128/JCM.41.4.1772-1774.2003

Prenatal diagnosis of congenital human cytomegalovirus infection in amniotic fluid by nucleic acid sequence-based amplification assay

J Clin Microbiol. 2003 Apr;41(4):1772-4. doi: 10.1128/JCM.41.4.1772-1774.2003.

Authors

M Grazia Revello¹, Daniele Lilleri, Maurizio Zavattoni, Milena Furione, Jaap Middeldorp, Giuseppe Gerna

Affiliation

¹ Servizio di Virologia, Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, 27100 Pavia, Italy.

Abstract

Nucleic acid sequence-based amplification assays for detection of human cytomegalovirus (HCMV) immediate-early and pp67 mRNA in 65 amniotic fluid samples tested for prenatal diagnosis of congenital HCMV infection showed sensitivity, specificity, and negative and positive predictive values >90%.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Amniotic Fluid / virology*
Cytomegalovirus / isolation & purification*
Cytomegalovirus Infections / congenital
Cytomegalovirus Infections / diagnosis*
Cytomegalovirus Infections / virology
Female
Fetal Diseases / diagnosis*
Fetal Diseases / virology
Humans
Predictive Value of Tests
Pregnancy
Prenatal Diagnosis*
Self-Sustained Sequence Replication*
Sensitivity and Specificity