Use of transgenic and mutant animal models in the study of heterocyclic amine-induced mutagenesis and carcinogenesis

J Biochem Mol Biol. 2003 Jan 31;36(1):35-42. doi: 10.5483/bmbrep.2003.36.1.035.

Abstract

Heterocyclic amines (HCAs) are potent mutagens generated during the cooking of meat and fish, and several of these compounds produce tumors in conventional experimental animals. During the past 5 years or so, HCAs have been tested in a number of novel in vivo murine models, including the following: lacZ, lacI, cII, c-myc/lacZ, rpsL, and gptDelta. transgenics, XPA-/-, XPC-/-, Msh2+/-, Msh2-/- and p53+/- knock-outs, Apc mutant mice (ApcDelta716, Apc1638N, Apcmin), and A33DeltaNbeta-cat knock-in mice. Several of these models have provided insights into the mutation spectra induced in vivo by HCAs in target and non-target organs for tumorigenesis, as well as demonstrating enhanced susceptibility to HCA-induced tumors and preneoplastic lesions. This review describes several of the more recent reports in which novel animal models were used to examine HCA-induced mutagenesis and carcinogenesis in vivo, including a number of studies which assessed the inhibitory activities of chemopreventive agents such as 1,2-dithiole-3-thione, conjugated linoleic acids, tea, curcumin, chlorophyllin-chitosan, and sulindac.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Adenomatous Polyposis Coli Protein / genetics
  • Amines / toxicity
  • Animals
  • Cell Transformation, Neoplastic / chemically induced*
  • Chemoprevention
  • DNA-Binding Proteins / genetics
  • Disease Models, Animal*
  • Heterocyclic Compounds / toxicity*
  • Mice
  • Mice, Knockout
  • Mice, Mutant Strains
  • Mice, Transgenic
  • MutS Homolog 2 Protein
  • Mutagenesis*
  • Neoplasms, Experimental / chemically induced*
  • Neoplasms, Experimental / genetics*
  • Proto-Oncogene Proteins / genetics
  • Ribosomal Proteins / genetics
  • Tumor Suppressor Protein p53 / genetics
  • Xeroderma Pigmentosum Group A Protein
  • beta-Galactosidase / genetics

Substances

  • Adenomatous Polyposis Coli Protein
  • Amines
  • DNA-Binding Proteins
  • Heterocyclic Compounds
  • Proto-Oncogene Proteins
  • Ribosomal Proteins
  • Tumor Suppressor Protein p53
  • Xeroderma Pigmentosum Group A Protein
  • Xpa protein, mouse
  • ribosomal protein S12
  • beta-Galactosidase
  • Msh2 protein, mouse
  • MutS Homolog 2 Protein