Unzipping dynamics of long DNAs

Phys Rev E Stat Nonlin Soft Matter Phys. 2002 Nov;66(5 Pt 1):051914. doi: 10.1103/PhysRevE.66.051914. Epub 2002 Nov 22.

Abstract

The two strands of the DNA double helix can be "unzipped" by the application of approximately 15 pN force. We analyze the dynamics of unzipping and rezipping for the case where the molecule ends are separated and reapproached at constant velocity. For unzipping of 50-kilobase DNAs at less than about 1000 bases per second, thermal-equilibrium-based theory applies. However, for higher unzipping velocities, rotational viscous drag creates a buildup of elastic torque to levels above k(B)T in the double-stranded DNA region, causing the unzipping force to be well above or well below the equilibrium unzipping force during, respectively, unzipping and rezipping, in accord with recent experimental results of Thomen et al. [Phys. Rev. Lett. 88, 248102 (2002)]. Our analysis includes the effect of sequence on unzipping and rezipping, and the transient delay in buildup of the unzipping force due to the approach to the steady state.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biophysical Phenomena
  • Biophysics
  • DNA / chemistry*
  • DNA, Single-Stranded / chemistry
  • Models, Chemical
  • Nucleic Acid Conformation
  • Nucleic Acid Denaturation
  • Thermodynamics

Substances

  • DNA, Single-Stranded
  • DNA