Acute myeloid leukemias with reciprocal rearrangements can be distinguished by specific gene expression profiles

Claudia Schoch; Alexander Kohlmann; Susanne Schnittger; Benedikt Brors; Martin Dugas; Susanne Mergenthaler; Wolfgang Kern; Wolfgang Hiddemann; Roland Eils; Torsten Haferlach

doi:10.1073/pnas.142103599

Acute myeloid leukemias with reciprocal rearrangements can be distinguished by specific gene expression profiles

Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):10008-13. doi: 10.1073/pnas.142103599. Epub 2002 Jul 8.

Authors

Claudia Schoch¹, Alexander Kohlmann, Susanne Schnittger, Benedikt Brors, Martin Dugas, Susanne Mergenthaler, Wolfgang Kern, Wolfgang Hiddemann, Roland Eils, Torsten Haferlach

Affiliation

¹ Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, University Hospital Grosshadern, Ludwig-Maximilians-University, 81366 Munich, Germany. claudia.schoch@med3.med.uni-muenchen.de

Abstract

Acute myeloid leukemia (AML) is a heterogeneous group of genetically defined diseases. Their classification is important with regard to prognosis and treatment. We performed microarray analyses for gene expression profiling on bone marrow samples of 37 patients with newly diagnosed AML. All cases had either of the distinct subtypes AML M2 with t(8;21), AML M3 or M3v with t(15;17), or AML M4eo with inv(16). Diagnosis was established by cytomorphology, cytogenetics, fluorescence in situ hybridization, and reverse transcriptase-PCR in every sample. By using two different strategies for microarray data analyses, this study revealed a unique correlation between AML-specific cytogenetic aberrations and gene expression profiles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acute Disease
Gene Expression Profiling*
Gene Rearrangement*
Humans
Leukemia, Myeloid / genetics*
Leukemia, Myeloid, Acute / classification*
Leukemia, Myeloid, Acute / genetics*
Models, Genetic
Oligonucleotide Array Sequence Analysis
Predictive Value of Tests
Reproducibility of Results