A polygalacturonase of animal origin isolated from the root-knot nematode Meloidogyne incognita

FEBS Lett. 2002 Jul 3;522(1-3):109-12. doi: 10.1016/s0014-5793(02)02906-x.

Abstract

The first animal polygalacturonase (PG, EC 2.1.15) encoding cDNA, Mi-pg-1, was cloned from the plant parasitic nematode Meloidogyne incognita. The enzymatic activity of MI-PG-1 was confirmed after heterologous expression in Escherichia coli. The presence of a predicted signal peptide on the MI-PG-1 sequence together with the specific localization of the transcripts of the Mi-pg-1 gene in the oesophageal glands of infective juveniles imply that MI-PG-1 could be secreted into plant tissues. The potential role of MI-PG-1 in parasitism is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Escherichia coli
  • Gene Expression
  • Genetic Vectors
  • Molecular Sequence Data
  • Phylogeny
  • Plant Roots / parasitology
  • Polygalacturonase / classification
  • Polygalacturonase / genetics*
  • Polygalacturonase / isolation & purification
  • Tylenchoidea / enzymology*
  • Tylenchoidea / genetics

Substances

  • Polygalacturonase

Associated data

  • GENBANK/AY098646