Target-assisted iterative screening reveals novel interactors for PSD95, Nedd4, Src, Abl and Crk proteins

J Biomol Struct Dyn. 2002 Jun;19(6):1015-29. doi: 10.1080/07391102.2002.10506805.

Abstract

A new in vitro screening method has been developed and applied to a commercial phage-displayed cDNA library to search for novel protein-protein interactions. PDZ, WW and SH3 domains from PSD95, Nedd4, Src, Abl and Crk proteins were used as targets. 12 novel putative and 2 previously reported interactions were identified in test screens. The novel screening format, dubbed TAIS (target-assisted iterative screening), is discussed as an alternative platform to existing technologies for a pair-wise characterization of protein-protein interactions.

MeSH terms

  • Amino Acid Sequence
  • Calcium-Binding Proteins / metabolism*
  • Chimerin Proteins / metabolism
  • Disks Large Homolog 4 Protein
  • Endosomal Sorting Complexes Required for Transport
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Ligases / metabolism*
  • Membrane Proteins / metabolism
  • Molecular Sequence Data
  • Myelin Proteins / metabolism
  • NAV1.2 Voltage-Gated Sodium Channel
  • Nedd4 Ubiquitin Protein Ligases
  • Nerve Tissue Proteins / metabolism*
  • Nogo Proteins
  • Oligopeptides / metabolism*
  • Protein Interaction Mapping
  • Protein Structure, Tertiary
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-abl / metabolism*
  • Proto-Oncogene Proteins c-crk
  • Recombinant Fusion Proteins / metabolism
  • Sequence Homology
  • Sodium Channels / metabolism
  • Ubiquitin-Protein Ligases*

Substances

  • Calcium-Binding Proteins
  • Chimerin Proteins
  • DLG4 protein, human
  • Disks Large Homolog 4 Protein
  • Endosomal Sorting Complexes Required for Transport
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Myelin Proteins
  • NAV1.2 Voltage-Gated Sodium Channel
  • Nerve Tissue Proteins
  • Nogo Proteins
  • Oligopeptides
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-crk
  • RTN4 protein, human
  • Recombinant Fusion Proteins
  • SCN2A protein, human
  • Sodium Channels
  • Src peptide
  • postsynaptic density proteins
  • LAPTM5 protein, human
  • Nedd4 Ubiquitin Protein Ligases
  • Nedd4 protein, human
  • Ubiquitin-Protein Ligases
  • Proto-Oncogene Proteins c-abl
  • Ligases