Differential regulation of the human and murine CD34 genes in hematopoietic stem cells

Yutaka Okuno; Hiromi Iwasaki; Claudia S Huettner; Hanna S Radomska; David A Gonzalez; Daniel G Tenen; Koichi Akashi

doi:10.1073/pnas.092027799

Differential regulation of the human and murine CD34 genes in hematopoietic stem cells

Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):6246-51. doi: 10.1073/pnas.092027799.

Authors

Yutaka Okuno¹, Hiromi Iwasaki, Claudia S Huettner, Hanna S Radomska, David A Gonzalez, Daniel G Tenen, Koichi Akashi

Affiliation

¹ Hematology/Oncology Division, Harvard Institutes of Medicine, and Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.

Abstract

Human CD34 (hCD34)-positive cells are used currently as a source for hematopoietic transplantation in humans. However, in steady-state murine hematopoiesis, hematopoietic stem cells (HSCs) with long-term reconstitution activity are found almost exclusively in the murine CD34 (mCD34)-negative to low fraction. To evaluate the possible differences in hCD34 and mCD34 gene expression in hematopoiesis, we made transgenic mouse strains with human genomic P1 artificial chromosome clones spanning the entire hCD34 genomic locus. In all transgenic mouse strains, a vast majority of phenotypic and functional HSC populations including mCD34(-/lo) express the hCD34 transgene. These data strongly support the notion that hCD34(+) human bone marrow cells contain long-term HSCs that can maintain hematopoiesis throughout life.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Antigens, CD34 / genetics*
Antigens, CD34 / metabolism
Cell Differentiation
Down-Regulation
Flow Cytometry
Gene Expression Regulation*
Genotype
Hematopoietic Stem Cells / metabolism*
Humans
Mice
Mice, Transgenic
Models, Biological
Models, Genetic
Phenotype
Species Specificity
Time Factors
Transgenes

Substances

Antigens, CD34