[Apoptosis of bone marrow cells aplastic anemia patients treated with cyclosporin A]

Zhonghua Xue Ye Xue Za Zhi. 2001 Apr;22(4):189-92.
[Article in Chinese]

Abstract

Objective: To evaluate the effects of cyclosporin A (CsA) on the apoptosis of bone marrow cells of aplastic anemia (AA) patients.

Methods: The apoptosis of and Fas antigen expression on the bone marrow mononuclear cells (MNC) in 25 AA patients and 10 normal controls were assayed by TUNEL and FACS, respectively.

Result: The percentage of CD(34)(+) cells was decreased significantly in AA patients than in normal controls (P < 0.05), and so did the percentage of CD(34)(+)Fas(+) cells (P < 0.05). The percentage of apoptotic cells in AA patients was higher than that of controls (P < 0.01). After the patients were treated with CsA for 2 months, the percentage of apoptotic cells was decreased (P < 0.01). The percentage of CD(34)(+)Fas(+) cells was positively correlated with that of apoptotic cells in AA patients.

Conclusion: Fas is involved in the apoptosis of CD(34)(+) cells of AA patients. CsA could reduce the percentage of apoptosis of bone marrow cells in AA patients.

Publication types

  • Clinical Trial
  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Anemia, Aplastic / drug therapy*
  • Anemia, Aplastic / physiopathology
  • Apoptosis / drug effects*
  • Bone Marrow Cells / cytology*
  • Bone Marrow Cells / drug effects
  • Child
  • Cyclosporine / therapeutic use*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Treatment Outcome
  • Young Adult

Substances

  • Cyclosporine