Opsonophagocytosis of fluorescent polystyrene beads coupled to Neisseria meningitidis serogroup A, C, Y, or W135 polysaccharide correlates with serum bactericidal activity

Joseph Martinez; Tamara Pilishvili; Suzanne Barnard; Joseph Caba; Willie Spear; Sandra Romero-Steiner; George M Carlone

doi:10.1128/cdli.9.2.485-488.2002

Opsonophagocytosis of fluorescent polystyrene beads coupled to Neisseria meningitidis serogroup A, C, Y, or W135 polysaccharide correlates with serum bactericidal activity

Clin Diagn Lab Immunol. 2002 Mar;9(2):485-8. doi: 10.1128/cdli.9.2.485-488.2002.

Authors

Joseph Martinez¹, Tamara Pilishvili, Suzanne Barnard, Joseph Caba, Willie Spear, Sandra Romero-Steiner, George M Carlone

Affiliation

¹ Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. jmartinez@cdc.gov

Abstract

We developed a polysaccharide-specific flow cytometric opsonophagocytic assay (OPA) for the simultaneous measurement of functional antibody to Neisseria meningitidis serogroups A, C, Y, and W135. OPA titers significantly correlated with serum bactericidal assay titers for all serogroups tested (mean r = 0.96; P < 0.001). OPA could be used in meningococcal vaccine evaluation.

MeSH terms

Adult
Antibodies, Bacterial / analysis*
Blood Bactericidal Activity / immunology*
Flow Cytometry / methods*
Fluorescent Dyes
HL-60 Cells
Humans
Meningococcal Infections / immunology*
Meningococcal Infections / prevention & control
Meningococcal Vaccines
Microspheres
Neisseria meningitidis / immunology*
Phagocytosis / immunology*
Polystyrenes
Serotyping

Substances

Antibodies, Bacterial
Fluorescent Dyes
Meningococcal Vaccines
Polystyrenes