Phage antibodies from combinatorial library neutralize vaccinia virus

N V Tikunova; V V Morozova; T A Batanova; E F Belanov; N I Bormotov; A A Ilyichev

Phage antibodies from combinatorial library neutralize vaccinia virus

Hum Antibodies. 2001;10(3-4):95-9.

Authors

N V Tikunova¹, V V Morozova, T A Batanova, E F Belanov, N I Bormotov, A A Ilyichev

Affiliation

¹ Research Center of Virology and Biotechnology, Vector, Koltsovo, Novosibirsk region, 630559, Russia. tikunova@vector.nsk.su

PMID: 11847420

Abstract

The library of human scFv antibodies displayed on the surface of bacteriophages was panned against Vaccinia virus (VACV), strain Elstree. 75% binding with Vaccinia virus. 5 clones were characterized for their binding with VACV and their ability to neutralize VACV in plaque reduction neutralization test (PRNT). Antibodies from the clones were obtained as soluble individual molecules and their binding activities were confirmed in ELISA.

MeSH terms

Antibodies, Viral / immunology*
Antibodies, Viral / isolation & purification
Combinatorial Chemistry Techniques
Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin Fragments / immunology*
Immunoglobulin Fragments / isolation & purification
Peptide Library
Recombinant Proteins / immunology
Vaccinia virus / immunology*

Substances

Antibodies, Viral
Immunoglobulin Fragments
Peptide Library
Recombinant Proteins
immunoglobulin Fv