[Expression of C3 epitope of poliovirus and a ten-peptides epitope of C-myc on the surface of recombinant bacteria]

Sheng Wu Gong Cheng Xue Bao. 2001 Sep;17(5):539-42.
[Article in Chinese]

Abstract

To confirm the possibility of pCSB136 and pCSX72 as vectors for displaying heterologous epitopes, the gene fragments coding for C3 epitope of poliovirus and a ten-peptides epitope of C-myc were synthesized and inserted into pCSB136 and pCSX72 respectively. The recombinants were screened by whole-strain PCR. The expression of recombinant proteins were detected by whole-cell ELISA and electronic microscopy. The results indicated the recombinant proteins were expressed as hybrid fimbriae, and the antigenicity of both CS3 and inserted epitopes kept. All results above showed vectors pCSB136 and pCSX72 could be used to display the foreign epitopes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsid / genetics*
  • Capsid Proteins
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / genetics
  • Escherichia coli / genetics*
  • Escherichia coli / ultrastructure
  • Gene Expression
  • Genes, myc / genetics*
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Microscopy, Electron
  • Poliovirus / genetics
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism

Substances

  • Capsid Proteins
  • Epitopes
  • Membrane Proteins
  • Recombinant Proteins
  • VP1 protein, Poliovirus