Differentiation of human hepatoma Hep G2 cells induced by 10-hydroxycamptothecin

X Zhang; Y Zhou; B Xu

Differentiation of human hepatoma Hep G2 cells induced by 10-hydroxycamptothecin

Chin Med J (Engl). 2000 Aug;113(8):712-3.

Authors

X Zhang¹, Y Zhou, B Xu

Affiliation

¹ Department of Pharmacology, Shanghai Institute of Material Medical, Chinese Academy of Sciences, Shanghai 200031, China.

PMID: 11776054

Abstract

Objective: To study the differentiation-inducing effect of hydroxycamptothecin (HCPT) on hepatoma Hep G2 cells.

Methods: 3-[4, 5-dimethylthial-2-yl]-3, 5-diphenyl-tetrazolinium bromide (MTT) colorimetric method was used for determining HepG2 cells viability. Alphafetoprotein (AFP) and albumin (ALB) levels were determined with Elisa Kits.

Results: The AFP secretion of HCPT-treated cells was reduced by 44.0%-49.2% while the ALB level was increased markedly.

Conclusion: Hydroxycamptothecin can induce human hepatoma Hep G2 cells to differentiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antineoplastic Agents, Phytogenic / pharmacology*
Camptothecin / analogs & derivatives*
Camptothecin / pharmacology*
Carcinoma, Hepatocellular / pathology*
Cell Differentiation / drug effects*
Humans

Substances

Antineoplastic Agents, Phytogenic
10-hydroxycamptothecin
Camptothecin