Fabrication of layer-by-layer deposited multilayer films containing DNA and its interaction with methyl green

L Luo; J Liu; Z Wang; X Yang; S Dong; E Wang

doi:10.1016/s0301-4622(01)00204-6

Fabrication of layer-by-layer deposited multilayer films containing DNA and its interaction with methyl green

Biophys Chem. 2001 Dec 11;94(1-2):11-22. doi: 10.1016/s0301-4622(01)00204-6.

Authors

L Luo¹, J Liu, Z Wang, X Yang, S Dong, E Wang

Affiliation

¹ Laboratory of Electroanalytical Chemistry and National Analytical Research Center of Electrochemistry and Spectroscopy, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.

PMID: 11744186
DOI: 10.1016/s0301-4622(01)00204-6

Abstract

Multilayer films were fabricated by layer-by-layer electrostatic deposition techniques between poly(diallyldimethylammonium chloride) (PDDA) and calf thymus DNA (CT DNA) on glassy carbon and quartz substrates. Electrochemical impedance spectroscopy (EIS), Fourier transform infrared (FTIR) spectroscopy and UV-vis spectroscopy demonstrated the uniform assembly of PDDA/DNA multilayer films, and X-ray photoelectron spectroscopy confirmed the elemental composition of the films. Moreover, the interaction of DNA in PDDA/DNA films with methyl green was investigated by UV-vis spectroscopy and circular dichroism (CD).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
DNA / chemistry*
Methyl Green / chemistry*
Spectrum Analysis
Static Electricity

Substances

Methyl Green
DNA