Assembly of microarrays for genome-wide measurement of DNA copy number

Nat Genet. 2001 Nov;29(3):263-4. doi: 10.1038/ng754.

Abstract

We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05-0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aneuploidy*
  • Chromosomes, Artificial, Bacterial / genetics
  • Cloning, Molecular
  • Female
  • Gene Dosage*
  • Genome, Human*
  • Genomics / methods*
  • Humans
  • Male
  • Oligonucleotide Array Sequence Analysis / methods*
  • Polymerase Chain Reaction
  • Polyploidy
  • Tumor Cells, Cultured
  • X Chromosome / genetics