Quantitating therapeutically relevant T-cell responses to cancer vaccines

Crit Rev Immunol. 2001;21(1-3):287-97.

Abstract

Successful application of active immunotherapy to the treatment of cancer will require stimulation of potent antigen-specific T-cell responses. It is not known how numerous or how potent these T cells must be in order to abrogate tumors, but the levels of immunity needed to control chronic viral infections may provide estimates for comparison. Evaluation of the efficacy of a vaccine strategy in attaining these levels of immunity will depend on the use of assays that create a picture of T-cell number and function that correlates with clinical outcomes. We discuss the currently available in vivo and in vitro T-cell assays and their relevance for detecting therapeutic levels of T-cell activity. We also propose a strategy for efficiently evaluating the immunologic efficacy of cancer vaccines so that the most promising candidates can be brought more rapidly into definitive clinical trials.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Cancer Vaccines / immunology*
  • Cytokines / analysis
  • Cytokines / genetics
  • Cytotoxicity Tests, Immunologic
  • Enzyme-Linked Immunosorbent Assay
  • Flow Cytometry
  • Humans
  • Hypersensitivity, Delayed
  • Lymphocyte Activation
  • RNA, Messenger / analysis
  • T-Lymphocytes / immunology*

Substances

  • Cancer Vaccines
  • Cytokines
  • RNA, Messenger