Alpha-oligonucleotides with anionic phosphodiester and cationic phosphoramidate linkages enhanced stability of DNA triple helix

F Ehrenmann; J J Vasseur; F Debart

doi:10.1081/NCN-100002432

Alpha-oligonucleotides with anionic phosphodiester and cationic phosphoramidate linkages enhanced stability of DNA triple helix

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):797-9. doi: 10.1081/NCN-100002432.

Authors

F Ehrenmann¹, J J Vasseur, F Debart

Affiliation

¹ Laboratoire de Chimie Organique Biomoléculaire de Synthèse, UMR 5625 CNRS-UM II, CC 008, Université Montpellier II, Place Eugène Bataillon, 34095 Montpellier, France.

PMID: 11563118
DOI: 10.1081/NCN-100002432

Abstract

Synthesis of several alpha-oligonucleotides containing both anionic phosphodiester and cationic N-(dimethylaminopropyl)phosphoramidate internucleosidic linkages is described. Their ability to form triple helix with dsDNA was evaluated at various pH by UV melting experiments.

MeSH terms

Amides / chemistry*
Cross-Linking Reagents / chemistry
DNA / chemistry*
Hydrogen-Ion Concentration
Nucleic Acid Conformation
Nucleic Acid Hybridization
Oligonucleotides / chemical synthesis
Oligonucleotides / chemistry*
Phosphoric Acids / chemistry*

Substances

Amides
Cross-Linking Reagents
Oligonucleotides
Phosphoric Acids
DNA
phosphoramidic acid