Abstract
We report the first synthetic peptide vaccine eliciting strong CD8(+) and CD4(+) T lymphocyte responses in humans. The vaccine, representing the C-terminal region of the circumsporozoite protein of Plasmodium falciparum (amino acids 282-383) was well tolerated and strong sporozoite-specific antibodies were elicited. In addition, robust lymphocyte proliferation responses were equally elicited with concomitant in vitro production of IFN-gamma, crucial in the elimination of the parasite. Most importantly, we also observed the development of CD8(+) T lymphocyte responses decisive in the immunity to malaria. The latter finding opens new, possibly safer, avenues for vaccination strategies when a CD8(+) T cell response is needed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Animals
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Antibodies, Protozoan / biosynthesis
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Antibody Specificity
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Antigens, Protozoan / immunology
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CD4-Positive T-Lymphocytes / immunology*
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CD8-Positive T-Lymphocytes / immunology*
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Cells, Cultured
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Female
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HLA-A Antigens / immunology
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Humans
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Immunologic Memory
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Interferon-gamma / biosynthesis
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Lymphocyte Activation
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Malaria Vaccines / adverse effects
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Malaria Vaccines / pharmacology*
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Malaria, Falciparum / immunology
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Malaria, Falciparum / therapy*
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Male
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Peptide Fragments / immunology*
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Peptides / immunology
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Plasmodium falciparum / immunology*
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Protozoan Proteins / immunology*
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Th1 Cells / immunology
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Vaccines, Synthetic / adverse effects
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Vaccines, Synthetic / pharmacology
Substances
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Antibodies, Protozoan
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Antigens, Protozoan
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HLA-A Antigens
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Malaria Vaccines
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Peptide Fragments
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Peptides
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Protozoan Proteins
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Vaccines, Synthetic
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circumsporozoite protein (282-383), Plasmodium falciparum
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circumsporozoite protein, Protozoan
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Interferon-gamma