Hybrid vectors based on adeno-associated virus serotypes 2 and 5 for muscle-directed gene transfer

J Virol. 2001 Jul;75(13):6199-203. doi: 10.1128/JVI.75.13.6199-6203.2001.

Abstract

Vectors based on hybrids consisting of adeno-associated virus types 2 (ITRs and Rep) and 5 (Cap) were evaluated for muscle-directed gene transfer (called AAV2/5). Evaluation in immune-competent mice revealed greater transduction efficacy with AAV2/5 than with AAV2 and no cross-neutralization between AAV2/5 and AAV2. Interestingly, we saw no immunologic evidence of previous exposure to AAV5 capsids in a large population of healthy human subjects.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Dependovirus / classification
  • Dependovirus / genetics*
  • Dependovirus / immunology
  • Genetic Therapy*
  • Genetic Vectors*
  • Humans
  • Hybridization, Genetic
  • Immunization
  • Mice
  • Mice, Inbred C57BL
  • Muscles / metabolism*
  • Serotyping

Substances

  • Antibodies, Viral