Anti-apoptotic effect of trans-resveratrol on paclitaxel-induced apoptosis in the human neuroblastoma SH-SY5Y cell line

Neurosci Lett. 2001 Apr 13;302(1):41-4. doi: 10.1016/s0304-3940(01)01654-8.

Abstract

Paclitaxel, an anticancer drug, induces apoptosis in human neuroblastoma cell line SH-SY5Y. The addition of trans-resveratrol, a natural antioxidant present in grapes and red wine, to SH-SY5Y cultures exposed to paclitaxel significantly reduces cellular death. The neuroprotective action of trans-resveratrol is due neither to its antioxidant capacity nor to interference with the polymerization of tubulin induced by paclitaxel. However, trans-resveratrol is able to inhibit the activation of caspase 7 and degradation of poly-(ADP-ribose)-polymerase which occur in SH-SY5Y exposed to paclitaxel. Resveratrol, therefore, exerts its anti-apoptotic effect by modulating the signal pathways that commit these neuronal-like cells to apoptosis.

MeSH terms

  • Antineoplastic Agents, Phytogenic / pharmacology
  • Antioxidants / pharmacology*
  • Apoptosis / drug effects*
  • Apoptosis / physiology
  • Caspase 7
  • Caspases / drug effects*
  • Caspases / metabolism
  • Humans
  • Neuroblastoma / metabolism*
  • Neuroblastoma / pathology
  • Paclitaxel / pharmacology
  • Resveratrol
  • Stilbenes / pharmacology*
  • Tumor Cells, Cultured / drug effects

Substances

  • Antineoplastic Agents, Phytogenic
  • Antioxidants
  • Stilbenes
  • CASP7 protein, human
  • Caspase 7
  • Caspases
  • Paclitaxel
  • Resveratrol