Comparison of IsoCode STIX and FTA Gene Guard collection matrices as whole-blood storage and processing devices for diagnosis of malaria by PCR

J Clin Microbiol. 2001 Mar;39(3):1195-6. doi: 10.1128/JCM.39.3.1195-1196.2001.

Abstract

We compared two collection devices, IsoCode and FTA, with whole blood for the diagnosis of malaria by PCR (n = 100). Using whole blood as the reference standard, both devices were sensitive for the detection of single-species malaria infections by PCR (> or =96%). However, the detection of mixed infections was suboptimal (IsoCode was 42% sensitive, and FTA was 63% sensitive).

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Specimen Collection / instrumentation*
  • Blood Specimen Collection / methods
  • DNA, Protozoan / blood*
  • Filtration
  • Humans
  • Malaria, Falciparum / complications
  • Malaria, Falciparum / diagnosis*
  • Malaria, Falciparum / parasitology
  • Malaria, Vivax / complications
  • Malaria, Vivax / diagnosis*
  • Malaria, Vivax / parasitology
  • Plasmodium falciparum / genetics
  • Plasmodium falciparum / isolation & purification
  • Plasmodium vivax / genetics
  • Plasmodium vivax / isolation & purification
  • Sensitivity and Specificity

Substances

  • DNA, Protozoan